Ocimum Tenuiflorum-mediated Biosynthesis of Silver Iron (Ag/Fe) Bimetallic Nanoparticles and Evaluation of their Antiglycation and Biocompatibility Activities

Abstract

Nano-biotechnology based revolution, which rests on the foundation laid by the green synthesis of nanoparticles (NPs), has given us hope to make significant developments in the area of biomedical science. A large number of biologically active constituents of plant extracts are involved not only in the production but also stabilization of NPs. This helps in making the overall process cost-effective along with being eco-friendly. The large variety of applications of FeNPs and AgNPs have drawn them a lot of attention lately. With the sudden emergence of antibiotic resistance and the suitable qualities of NPs, scientists and researchers have started building connections more than ever. In this study, we synthesized Ag/Fe Bimetallic Nanoparticles from the Ocimum Tenuiflorum plant extract. The Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) of the fresh leaves of plant extract were determined to find out their antioxidant potential. UV-Visible spectrophotometry of green synthesized Ag/Fe Bimetallic NPs presented a distinctive band of surface Plasmon resonance within the range of 380-420 nm. The synthesized NPs were characterized using Fourier Transform Infrared spectroscopy and X-Ray Diffraction analysis techniques. Synthesized NPs were checked for their biological activities such as anti-glycation and biocompatibility activities. FTIR showed the presence of phenols, aliphatic and aromatic hydrocarbons and flavonoid compounds that were used in bioreduction and capping of Ag/Fe NPs. XRD was conducted to determine the particle size and crystalline nature of both types of NPs. The Ag/Fe bimetallic nanoparticles had spherical shape and a particle size of 13 nm. In case of antiglycation and biocompatibility activities results showed their high potential in clinically multi-drug resistant strains, to develop synergistic combinatorial activities with possible applications in both industrial processes and the treatment of infections caused by clinically relevant drug resistant strains. This study is distinct because Ag/Fe Bimetallic NPs from ocimum tenuiflorum have not been synthesized, characterized and their biological activities have not been evaluated.