http://pjbmb.com/index.php/pjbmb/issue/feed 2025-10-05T15:30:43+00:00 Prof. Dr. M. Kamran Azim exeditor.pjbmb@gmail.com Open Journal Systems <div class="fw-row"> <div class="fw-col-xs-12"> <div class="seline-default-heading"> <p>The Pakistan Journal of Biochemistry and Molecular Biology (PJBMB) (ISSN P 1681-4525; ISSN E 2788-4147) is an open-access journal that publishes papers that contribute to knowledge in the field of Biochemistry, Molecular Biology, and related areas.</p> <p>PJBMB is currently published under the auspices of Mohammad Ali Jinnah University in Karachi, Pakistan.</p> <p>An article for publication must describe substantial original research undertaken according to the scope of this journal. A manuscript submitted for publication implies that the work described has not been published before (except as an abstract or a published lecture, review, or thesis). At the time of its submission to PJBMB it should neither be under consideration for publication elsewhere nor will it be reproduced after its publication in any other journal. Any co-authors must have agreed with the contents of the publication including the institution where the work was carried out. All authors should have agreed to the automatic transfer of copyright to the Editor-in-Chief of PJBMB.</p> <p>Since 1968, the Pakistan Journal of Biochemistry and Molecular Biology (PJBMB) has been consistently published as the official publication of the Pakistan Society of Biochemistry and Molecular Biology. As a result, it stands as one of the nation’s most longstanding scholarly periodicals.</p> <p>Since 2010, volumes of PJBMB is being published online (ISSN E 2788-4147). Recently, PJBMB has established online journal publishing with the help of "Open Journal Systems" (OJS). The OJS is an open-source software application for managing and publishing scholarly journals.</p> <p>PJBMB volumes published since 2018 to date are available online at pjbmb.com. Whereas, volumes published during 2010-2017 can be accessed at www.pjbmb.org.pk.</p> </div> </div> </div> http://pjbmb.com/index.php/pjbmb/article/view/132 2025-03-27T09:18:16+00:00 Muhammad Zubair muhammadzubair@gmail.com Aroofa Tasneem adeenashariya@gmail.com Sahrish Haji sahrishhaji1122@gmail.com Umar Siddique umar.siddique@tih.org.pk

<p>Timely reporting of critical laboratory results is crucial for patient safety and clinical decision-making. Critical alerts, also known as panic values, necessitate immediate communication with clinicians to enable prompt intervention. This study evaluates the frequency and timeliness of critical alert reporting as a key performance indicator in a specialized kidney institute's clinical laboratory. A retrospective cross-sectional study was conducted at the Multan Institute of Kidney Diseases over 2.5 years (April 2021–October 2023). Data on critical alerts were extracted from the Laboratory Information System (LIS) and analyzed using Microsoft Excel. The study assessed the incidence of critical values across different lab sections and evaluated adherence to the 30-minute reporting benchmark. A total of 1,015,285 tests were processed, with 18,540 (2.5%) yielding critical values. The majority of alerts originated from chemical pathology (83.4%), followed by hematology (16.6%). Despite a stringent reporting target of 30 minutes, the compliance rate was high, with hematology achieving 99.82% and chemical pathology 96.72% of timely notifications. Serum electrolytes, creatinine, and complete blood count (CBC) tests were the most frequently flagged parameters. Notably, the outpatient department (OPD) accounted for the highest number of critical alerts due to higher test requisition rates. The study highlights the effectiveness of structured protocols and digital tools in enhancing the timeliness of critical result reporting. While the laboratory demonstrated high compliance with CAP-recommended reporting timeframes, occasional delays were attributed to system workload and recipient response time. Continuous staff training and the integration of automated alert systems could further optimize reporting efficiency and patient care.</p>

2025-10-05T00:00:00+00:00 Copyright (c) 2025 PAKISTAN JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY http://pjbmb.com/index.php/pjbmb/article/view/131 2025-01-20T11:38:45+00:00 Seerat Fatima seeratf263@gmail.com Nimra Arshad Arshadnimra251@gmail.com

<p>Nano-biotechnology based revolution, which rests on the foundation laid by the green synthesis of nanoparticles (NPs), has given us hope to make significant developments in the area of biomedical science. A large number of biologically active constituents of plant extracts are involved not only in the production but also stabilization of NPs. This helps in making the overall process cost-effective along with being eco-friendly. The large variety of applications of FeNPs and AgNPs have drawn them a lot of attention lately. With the sudden emergence of antibiotic resistance and the suitable qualities of NPs, scientists and researchers have started building connections more than ever. In this study, we synthesized Ag/Fe Bimetallic Nanoparticles from the <em>Ocimum Tenuiflorum </em>plant extract. The Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) of the fresh leaves of plant extract were determined to find out their antioxidant potential. UV-Visible spectrophotometry of green synthesized Ag/Fe Bimetallic NPs presented a distinctive band of surface Plasmon resonance within the range of 380-420 nm. The synthesized NPs were characterized using Fourier Transform Infrared spectroscopy and X-Ray Diffraction analysis techniques. Synthesized NPs were checked for their biological activities such as anti-glycation and biocompatibility activities. FTIR showed the presence of phenols, aliphatic and aromatic hydrocarbons and flavonoid compounds that were used in bioreduction and capping of Ag/Fe NPs. XRD was conducted to determine the particle size and crystalline nature of both types of NPs. The Ag/Fe bimetallic nanoparticles had spherical shape and a particle size of 13 nm. In case of antiglycation and biocompatibility activities results showed their high potential in clinically multi-drug resistant strains, to develop synergistic combinatorial activities with possible applications in both industrial processes and the treatment of infections caused by clinically relevant drug resistant strains. This study is distinct because Ag/Fe Bimetallic NPs from <em>ocimum tenuiflorum </em>have not been synthesized, characterized and their biological activities have not been evaluated.</p>

2025-10-05T00:00:00+00:00 Copyright (c) 2025 PAKISTAN JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY