Potent Antibacterial Activity of Proteins Isolated from Acacia Honey

Authors

  • Rabia Irshad Jinnah Postgraduate Medical Centre, Karachi, Pakistan.

Keywords:

Antibacterial activity; Honey protein; HPLC; Gel Filtration Chromatography

Abstract

Antibacterial resistance to antibiotics has become a global health issue. Therefore, alternative antimicrobial strategies are urgently needed. The use of alternative remedies such as plant-based products might be effective. Natural honey has been used as an antibacterial agent against many bacterial species. In this study, the antibacterial activities of natural honey and honey proteins were tested against six pathogenic bacteria. Honey proteins were extracted by ammonium sulfate and acetone followed by protein estimation the Lowry method. SDS-PAGE was used to determine the molecular weight of honey proteins. Extracted honey proteins were separated by Gel Filtration Chromatography using HPLC. Antibacterial activity of the total honey protein was performed by broth microdilution method and IC50 was determined. The Acacia honey showed potent antibacterial activity with IC50 of 45 ng/µL to 55 ng/µL against bacterial isolates. The GFC fractionated protein (peak 3) of Acacia honey inhibited the growth of E. coli (86%), P. aeruginosa (79%), S. typhi (88%), and S. aureus (87%).

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Published

2024-11-24

How to Cite

Irshad, R. (2024). Potent Antibacterial Activity of Proteins Isolated from Acacia Honey. PAKISTAN JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 57(1), 37–55. Retrieved from https://pjbmb.com/index.php/pjbmb/article/view/119