2D gel electrophoresis and HPLC separation profile of Echis carinatus sochureki snake venom

Abstract

Snakebite envenomation is a neglected tropical disease. In Pakistan, the venomous snakes responsible for high morbidity particularly in rural areas includes Echis carinatus, Naja naja, Daboia russelii, and Bungarus species. Our study presents HPLC and gel-based de-complexation of proteins and peptides from the venom of Echis carinatus sochureki, commonly called as saw-scaled viper. The combined analysis showed the presence of proteins in the range of 95-22 KDa as a major portion while the high and low molecular mass proteins were found to be in less abundance. The 2D gel showed the presence of acidic to basic range proteins. The 2D gel also reveals that the low molecular mass proteins are mostly at the acidic side.