The Study of Stress and Depression Measurements Using HPLC as a Diagnostic Tool: Behavioural and Instrumental-Based Study

Stress And Depression Measurements

Abstract

Stress is a bizarre mental and emotional condition in reaction to bodily or physiological pressure. It is taken into consideration to be a primary element in inducing anxiety and depression which in turn can result in various health problems. It is regarded as activating the vital serotonergic gadget. Beneath physiological conditions, the level of serotonin (5-hydroxytryptamine: 5-HT) synthesis generally depends on the concentration of its precursor tryptophan (TRP). The physiological thing of stress alters the supply of TRP inside the brain and thereby affects the charge of 5-HT synthesis there. In this study, high-performance liquid chromatography (HPLC) was used as a diagnostic tool to monitor stress and despair responses in terms of TRP concentration. Because stress and depression are usually diagnosed through changes in a person's physical activity and behavior. Therefore, in addition to the instrument-based study, a behavioral study was also conducted. The prevalence of stress in the target population was studied on a biochemical and behavioral basis. The results of our study show that students who do not interact socially are more vulnerable to stress than students who do interact socially. Changes in TRP concentrations are suggested as an accurate and reliable biochemical basis for stress detection.